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liaR [2020-05-04 09:09:01]
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liaR [2020-05-04 09:09:01]

two-component response regulator, regulation of the liaI-liaH-liaG-liaF-liaS-liaR operon in response to bacitracin
Locus
BSU_33080
Isoelectric point
4.96
Molecular weight
22.98 kDa
Protein length
211 aa Sequence Blast
Gene length
636 bp Sequence Blast
Function
regulation of the liaI-liaH-liaG-liaF-liaS-liaR operon
Product
two-component response regulator
Essential
no
Synonyms
yvqC

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,394,422 3,395,057

The protein

Catalyzed reaction/ biological activity

  • regulation of the liaI-liaH-liaG-liaF-liaS-liaR operon in response to bacitracin
  • Paralogous protein(s)

    Domains

  • Response regulatory domain (aa 3-119) (according to UniProt)
  • HTH luxR-type domain (aa 143-208) (according to UniProt)
  • Modification

  • phosphorylation on a Asp residue by LiaS
  • Structure

  • 5HEV (LiaR from Enterococcus faecium, 62% identity) PubMed
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • LiaR: activation, PubMed, in LiaR regulon
  • Regulation

  • liaG: constitutive
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    Description

    Regulation

  • liaG: constitutive
  • view in new tab

    Biological materials

    Mutant

  • MGNA-B034 (yvqC::erm), available at the NBRP B. subtilis, Japan
  • BKE33080 (liaR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCCCATTCTGACCATTTCAT, downstream forward: _UP4_AATCATCTCGTGAATTAGGC
  • BKK33080 (liaR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCCCATTCTGACCATTTCAT, downstream forward: _UP4_AATCATCTCGTGAATTAGGC
  • Labs working on this gene/protein

  • John Helmann, Cornell University, USA Homepage
  • References

    Reviews

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    Original publications

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